Assistant Professor Biography A native of Florida, Kelly completed her undergraduate studies in Chemistry at the University of Florida. After graduating with honors in 2009, Kelly joined the Department of Chemistry at Vanderbilt University as a graduate student. Her research in the lab of John A. McLean focused on the development of ion mobility-mass spectrometry (IM-MS) methods for the identification of metabolite, lipid, and peptide biomolecular signatures of disease from complex biological samples. After receiving her Ph.D. in 2014, Dr. Hines completed a one-year postdoctoral fellowship in the Mayo Clinic Regional Metabolomics Core where she established quantitative MS methods for lipidomics and protein metabolism using isotope labeling. In 2015, Dr. Hines joined the lab of Libin Xu at the University of Washington. Her work in the Xu Lab focused on the development of IM-MS methods for lipidomics, high-throughput IM-MS measurements of drugs and small molecules, and the significance of lipids in human diseases, environmental exposure and antibiotic resistance. Kelly joined the Department of Chemistry at University of Georgia as an Assistant Professor in 2019. Education Education: Ph.D. in Chemistry (2014), Vanderbilt University B.S. in Chemistry (2009), University of Florida Research Research Areas: Analytical Chemistry Biological Chemistry Research Interests: Our goal is to better understand the ways in which lipids and small molecules contribute to diseases affecting human health and use this knowledge to develop diagnostic and prognostic tools based on molecular signatures of the disease. To achieve this, the Hines Lab develops and applies bioanalytical methods using ion mobility-mass spectrometry (IM-MS) to enhance the throughput and dimensionality of lipidomics and metabolomics experiments. One focus of the group is antibiotic resistance, where we are characterizing the metabolic alterations in antibiotic resistant pathogens and developing IM-MS methods for antibiotic susceptibility testing and small molecule screening. Selected Publications Selected Publications: #Corresponding Author Christian D. Freeman, Tayte Hansen, Ramona Urbauer, Brian J. Wilkinson, Vineet K. Singh, and Kelly M. Hines#, “Defective pgsA contributes to increased membrane fluidity and cell wall thickening in S. aureus with high-level daptomycin resistance,” mSphere 2024, 0:e00115-24. Hannah M. Hynds and Kelly M. Hines#, “MOCCal: A Multi-Omic CCS Calibrator for Traveling Wave Ion Mobility Mass Spectrometry,” Analytical Chemistry, 2024, 96, 1185-1194. Jana M. Carpenter, Hannah M. Hynds, Kingsley Bimpeh, and Kelly M. Hines#, “HILIC-IM-MS for Simultaneous Lipid and Metabolite Profiling of Bacteria,” ACS Measurement Science Au, 2024, 4(1), 104-116. Invited contribution for 2023 Rising Stars in Measurement Science virtual special issue. Kingsley Bimpeh and Kelly M. Hines#, “A rapid single-phase extraction for polar staphylococcal lipids,” Analytical and Bioanalytical Chemistry, 2023, 415, 4591–4602. Invited contribution for Young Investigators in (Bio-)Analytical Chemistry 2023 Collection. Hannah M. Hynds and Kelly M. Hines#, "Ion Mobility Shift Reagents for Lipid Double Bonds Based on Paternò–Büchi Photoderivatization with Halogenated Acetophenones," Journal of the American Society for Mass Spectrometry, 2022, DOI: 10.1021/jasms.2c00211 Katherine E. Havranek, Judith M.R. Ballista, Kelly M. Hines#, and Melinda A. Brindley#, "Untargeted Lipidomics of Vesicular Stomatitis Virus-Infected Cells and Viral Particles," Viruses, 2022, DOI: 10.3390/v14010003. Christian Freeman, Hannah M. Hynds, Jana M. Carpenter, Keerthi Appala, Kingsley Bimpeh, Shannon Barbarek, Craig Gatto, Brian J. Wilkinson, and Kelly M. Hines#, “Revealing Fatty Acid Heterogeneity in Staphylococcal Lipids with Isotope Labeling and RPLC-IM-MS,” Journal of the American Society for Mass Spectrometry, 2021, DOI:10.1021/jasms.1c00092. Invited contribution for Emerging Investigators Special Focus Issue. Kelly M. Hines, Gloria Alvarado, Xi Chen, Craig Gatto, Antje Pokorny, Francis Alonzo III, Brian J. Wilkinson, and Libin Xu, “Lipidomic and Ultrastructural Characterization of the Cell Envelope of Staphylococcus aureus Grown in the Presence of Human Serum,” mSphere, 2020, 5:e00339-20. Keerthi Appala*, Kingsley Bimpeh*, Christian Freeman*, and Kelly M. Hines#, “Recent applications of mass spectrometry in bacterial lipidomics,” Analytical and Bioanalytical Chemistry, 2020, 412, 5935–5943. Invited contribution for Female Role Models in Analytical Chemistry Special Issue. *These authors contributed equally. Kelly M. Hines, Tianwei Shen, Nathaniel K. Ashford, Adam Waalkes, Kelsi Penewit, Elizabeth A. Holmes, Kathryn McLean, Stephen J. Salipante, Brian J. Werth, Libin Xu, “Occurrence of cross-resistance and β-lactam seesaw effect in glycopeptide-, lipopeptide- and lipoglycopeptide-resistant MRSA correlates with membrane phosphatidylglycerol levels,” Journal of Antimicrobial Chemotherapy, 2020, dkz562. Kelly M. Hines and Libin Xu, “Lipidomic consequences of phospholipid synthesis defects in Escherichia coli revealed by HILIC-ion mobility-mass spectrometry,” Chemistry and Physics of Lipids, 2019, 219, 15-22. Kelly M. Hines, Adam Waalkes, Kelsi Penewit, Elizabeth A. Holmes, Stephen J. Salipante, Brian J. Werth, Libin Xu, “Characterization of the Mechanisms of Daptomycin Resistance among Gram-Positive Bacterial Pathogens by Multidimensional Lipidomics,” mSphere, 2017, 2 (6), e00492-17. Kelly M. Hines, Dylan H. Ross, Kimberly L. Davidson, Matthew F. Bush, Libin Xu, “Large-Scale Structural Characterization of Drug and Drug-like Compounds by High-Throughput Ion Mobility-Mass Spectrometry,” Analytical Chemistry, 2017, 89 (17), 9023-9030. Kelly M. Hines, Josi Herron, Libin Xu, “Assessment of altered lipid homeostasis by HILIC-ion mobility-mass spectrometry-based lipidomics,” Journal of Lipid Research, 2017, 58, 809-819. Kelly M. Hines, Jody C. May, John A. McLean, Libin Xu, “Evaluation of Collision Cross Section Calibrants for Structural Analysis of Lipids by Traveling Wave Ion Mobility-Mass Spectrometry,” Analytical Chemistry, 2016, 88 (14), 7329-7336. #Corresponding Author For a current list: Google Scholar Of note: 2024 Chemist of the Year, Northeast Georgia Chapter of the American Chemical Society 2023 ASMS Research Award 2023 Member-at-Large for Publications for the American Society for Mass Spectrometry 2021 Emerging Investigator, Journal of the American Society for Mass Spectrometry 2020 Female Role Model in Analytical Chemistry, Analytical and Bioanalytical Chemistry
