Education
Ph.D. Vanderbilt University 2014
B.S. University of Florida 2009
Research
Our goal is to better understand the ways in which lipids and small molecules contribute to diseases affecting human health and use this knowledge to develop diagnostic and prognostic tools based on molecular signatures of the disease. To achieve this, the Hines Lab develops and applies bioanalytical methods using ion mobility-mass spectrometry (IM-MS) to enhance the throughput and dimensionality of lipidomics and metabolomics experiments. One focus of the group is antibiotic resistance, where we are characterizing the metabolic alterations in antibiotic resistant pathogens and developing IM-MS methods for antibiotic susceptibility testing and small molecule screening.
Selected Publications
Christian Freeman, Hannah M. Hynds, Jana M. Carpenter, Keerthi Appala, Kingsley Bimpeh, Shannon Barbarek, Craig Gatto, Brian J. Wilkinson, and Kelly M. Hines, “Revealing Fatty Acid Heterogeneity in Staphylococcal Lipids with Isotope Labeling and RPLC-IM-MS,” Journal of the American Society for Mass Spectrometry, 2021, DOI:10.1021/jasms.1c00092. Invited contribution for Emerging Investigators Special Focus Issue.
Kelly M. Hines, Gloria Alvarado, Xi Chen, Craig Gatto, Antje Pokorny, Francis Alonzo III, Brian J. Wilkinson, and Libin Xu, “Lipidomic and Ultrastructural Characterization of the Cell Envelope of Staphylococcus aureus Grown in the Presence of Human Serum,” mSphere, 2020, 5:e00339-20.
Keerthi Appala*, Kingsley Bimpeh*, Christian Freeman*, and Kelly M. Hines, “Recent applications of mass spectrometry in bacterial lipidomics,” Analytical and Bioanalytical Chemistry, 2020, 412, 5935–5943. Invited contribution for Female Role Models in Analytical Chemistry Special Issue. *These authors contributed equally.
Kelly M. Hines, Tianwei Shen, Nathaniel K. Ashford, Adam Waalkes, Kelsi Penewit, Elizabeth A. Holmes, Kathryn McLean, Stephen J. Salipante, Brian J. Werth, Libin Xu, “Occurrence of cross-resistance and β-lactam seesaw effect in glycopeptide-, lipopeptide- and lipoglycopeptide-resistant MRSA correlates with membrane phosphatidylglycerol levels,” Journal of Antimicrobial Chemotherapy, 2020, dkz562.
Kelly M. Hines and Libin Xu, “Lipidomic consequences of phospholipid synthesis defects in Escherichia coli revealed by HILIC-ion mobility-mass spectrometry,” Chemistry and Physics of Lipids, 2019, 219, 15-22.
Kelly M. Hines, Adam Waalkes, Kelsi Penewit, Elizabeth A. Holmes, Stephen J. Salipante, Brian J. Werth, Libin Xu, “Characterization of the Mechanisms of Daptomycin Resistance among Gram-Positive Bacterial Pathogens by Multidimensional Lipidomics,” mSphere, 2017, 2 (6), e00492-17.
Kelly M. Hines, Dylan H. Ross, Kimberly L. Davidson, Matthew F. Bush, Libin Xu, “Large-Scale Structural Characterization of Drug and Drug-like Compounds by High-Throughput Ion Mobility-Mass Spectrometry,” Analytical Chemistry, 2017, 89 (17), 9023-9030.
Kelly M. Hines, Josi Herron, Libin Xu, “Assessment of altered lipid homeostasis by HILIC-ion mobility-mass spectrometry-based lipidomics,” Journal of Lipid Research, 2017, 58, 809-819.
Kelly M. Hines, Jody C. May, John A. McLean, Libin Xu, “Evaluation of Collision Cross Section Calibrants for Structural Analysis of Lipids by Traveling Wave Ion Mobility-Mass Spectrometry,” Analytical Chemistry, 2016, 88 (14), 7329-7336.
For a current list: https://scholar.google.com/citations?user=vA9jfvcAAAAJ
