Tags: Analytical Seminar


Characterization of the Interaction of the Regulator of G-protein Signaling 10 (RGS10) and the Calcium Signaling Protein Calmodulin

Characterization of the interaction of the regulator of G-protein signaling 10 (RGS10) and the calcium signaling protein calmodulin G-Protein Coupled Receptors (GPCRs) are of high therapeutic importance, being the targets of approximately 35% of all drugs currently in clinical use. G-Protein activation upon agonist binding to a GPCR is mediated by the exchange of GDP to GTP in the G𝛼 subunit of the heterotrimeric G-Protein, which causes the…


Diagnosis of Infectious Diseases by Ion Mobility Mass Spectrometry

There is a projection of 10 million deaths per year by 2050 due to antimicrobial resistance, highlighting the need for rapid detection and accurate identification of microorganisms. Therefore, developing fast robust diagnosis techniques will increase the recovery rates of patients suffering from various infections and will lead to significantly reduced antibiotic resistance. The membrane of bacteria contains varying lipid compositions that can…


Multi-Dimensional Separations for Bacteria Identifications and Investigations of Antimicrobial Resistance

Bacterial infections are a major global health concern, with an estimated 1 in 8 deaths attributed to bacterial infections in 2019 alone. When a patient with a suspected infection arrives in a clinical setting, the first step to developing a treatment strategy is to identify the causative pathogen, followed by determination of antimicrobial therapeutics that are appropriate and effective. The challenge for both these essential steps is the…


Imaging Biological Tissues at High Chemical and Spatial Resolutions Using Mass Spectrometry

Imaging mass spectrometry is a powerful analytical technique for analyzing the spatial lipidome. This technology enables the visualization of molecular pathology directly in tissues by combining the specificity of mass spectrometry with the spatial fidelity of microscopic imaging. This label-free methodology has proven exceptionally useful in research areas such as cancer diagnosis, diabetes, and infectious disease. However, state-of-the-art…


Two Tales of Measuring More than Mass with Mass Spectrometers: Differentiating Hydroxyproline Isomers and Understanding Ionic Liquid Degradation

We are broadly interested in using mass spectrometry-based platforms to learn more than just the mass about chemical species. During this talk two examples of this will be presented. In the first, infrared ion spectroscopy will be presented as one path toward direct structural probing of gas-phase ions, with the goal of isomer differentiation. Hydroxyproline isomers will be presented as a case study toward this effect. In the second, our work to…


In vitro Insights of Exogenous Fatty acids and FASII Inhibitors on Lipid Profiles and Antibiotic Susceptibilities in Staphylococcus aureus

The prevalence of hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) infections is on the rise, primarily due to the rapid development of resistance mechanisms by S. aureus against commonly used antibiotics.1 Daptomycin, the last-resort antibiotic for MRSA, has encountered challenges as studies reveal acquired resistance by S. aureus.2 FASII is a distinct metabolic pathway associated in synthesis of fatty acids through a series…


Connecting Branched Isomer Distributions and Membrane Characteristics in Daptomycin-Resistant Staphylococcus aureus Using RPLC-IM-MS

Staphylococcus aureus is a common cause of hospital and community-acquired infections. Although uncommon, resistance to daptomycin is mediated by mutations in genes associated with the regulation, synthesis, or relocation of fatty acids and membrane lipids. These mutations influence physical properties such as cell surface charge, cell membrane fluidity, and daptomycin resistance, however, recent results were unable to provide an in-depth…


The MAW Method: A Rapid Single-Phase Extraction for Bacterial Lipidomics

The increasing incidence of antibiotic resistance underscores the pressing need for studies aimed at understanding its underlying mechanisms. Bacterial membrane lipids provide an avenue for studies, as they are crucial for maintaining the integrity and functionality of bacterial cell membranes. However, in-depth studies on the involvement of bacterial membrane lipids require working with numerous bacterial samples, which necessitates the need…


Intrinsically Disordered Proteins (IDPs): NMR Studies of IDPs

Intrinsically disordered proteins (IDPs) - polypeptides that lack a defined three-dimensional structure – are commonplace in eukaryotic proteomes and often have critical impact on correct functioning of biological processes. IDPs can mediate low affinity interactions between proteins and serve as a scaffold or intermediate binding agent. Malfunctions in IDPs can lead to protein aggregation and various diseases, such as Alzheimer's, Parkinson’s,…


Direct Mass Spectrometry Technologies for Intraoperative Tissue Analysis and Surgical Margin Evaluation

Surgical resection remains a primary treatment for solid tumors. However, achieving tumor-free margins during surgery is challenging. Residual cancer cells at surgical margins are associated with increased recurrence and reduced survival rates. Intraoperative evaluation of surgical margins could enable more complete resection and reduce positive margins. Traditional frozen section histopathology has limitations including time-consumption,…
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