Tags: Analytical Seminar


Developing Ultrasensitive Library-Aliquot-based Droplet Digital PCR for detecting T790M

Patients with non-small cell lung cancer (NSCLC) harboring activating mutations in the epidermal growth factor receptor (EGFR) show significant progression-free survival benefit when they are treated with EGFR tyrosine kinase inhibitors (TKIs), have been recommended as the first-line therapy for NSCLC patents with EGFR-sensitizing mutations.1 Unfortunately, the vast majority acquire resistance after 9-12 months of treatment. However, the…


Living Single-Cell Real Time Imaging via Plasmonic-Enhanced Rayleigh & Raman Spectroscopy

Single-cell analysis is an active research area focused on deciphering cellular processes from a single living cell.1 Various crucial biological phenomena are either invisible or only partially characterized when analyzed with standard techniques that utilize bulk cell populations or entire tissues. This is due to the high level of heterogeneity within cell cultures.1-3 To provide novel insight into these diverse biological properties specific…


TBA


Fabric Phase Sorptive Extraction on Emerging Contaminants in Environmental Waters

Emerging contaminants in water is a major focus of many environmental and public health organizations worldwide.1-2 Specifically, emerging contaminants of concern are pharmaceuticals such as over the counter drugs, hormonal supplements, and veterinary medicines. It is known that trace amounts of these chemicals are present in drinking water and in surface water. These chemicals have adverse effects on wildlife and the subsequent ecosystems, and…


Versatile Polymer-based Glycosurface Fabrications for Gliding Motility of Mycoplasma Pneumoniae

Glycopolymer is an important class of synthetic molecules that emulate many biological and structural characteristics of cell-surface glycoproteins or glycolipids. Here, a general and versatile strategy is reported for the fabrication of glycopolymer-modified substrates that ligates reducing glycans toward polymeric scaffold through hydrazide linker.1 The fabrication process and conjugation proceeded in mild conditions with good ligation…


Negative Electron Transfer Dissociation paired with Capillary Electrophoresis-Mass Spectrometry for the investigation of Glycosaminoglycan mixtures

Sulfated glycosaminoglycan (GAG) carbohydrates are long, linear, acidic polysaccharide chains abundant on the surface of virtually all mammalian cells[1,2]. Non-template driven modifications affect many biological functions through protein-binding interactions[3]. The complex structure and low natural abundance of GAG oligomers remains a significant analytical challenge. Capillary electrophoresis-mass spectrometry (CE-MS) presents an efficient…


Quantification of HIV-1 RNA in dried blood spots using real time quantitative PCR in resource limited settings

Despite medical advancements to eradicate human immunodeficiency virus (HIV) around the world, HIV is still among the top leading causes of death in untreated newborn in resourcelimited countries. To address this issue, a confirmatory HIV infection test is required as soon as the baby is born to benefit from available HIV therapies such as antiretroviral therapy1.  However, the presence of maternal HIV antibodies transferred during…


Plasmonic Fluorescence Enhancement in Single Molecule-Based Detection with Nanoantenna

Over the last decade, the field of single-molecule detection has grown rapidly because technical and methodological developments increased sensitivity and gave access to biological processes not observable before. Single molecule detection unveils the short-lived intermediates, the heterogeneous behavior of individual enzymes and stochastic multistep processes like protein folding.1 Currently, single-molecule detection mostly relies on…


Threshold Activation Tandem Mass Spectrometry Coupled to Capillary Electrophoresis for the Structural Analysis of Glycosaminoglycan Mixtures


Explanation Construction and Affective Characteristics in an Active Learning Preparatory Chemistry Course

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