Tags: Analytical Seminar


Negative Electron Transfer Dissociation paired with Capillary Electrophoresis-Mass Spectrometry for the investigation of Glycosaminoglycan mixtures

Sulfated glycosaminoglycan (GAG) carbohydrates are long, linear, acidic polysaccharide chains abundant on the surface of virtually all mammalian cells[1,2]. Non-template driven modifications affect many biological functions through protein-binding interactions[3]. The complex structure and low natural abundance of GAG oligomers remains a significant analytical challenge. Capillary electrophoresis-mass spectrometry (CE-MS) presents an efficient…


Quantification of HIV-1 RNA in dried blood spots using real time quantitative PCR in resource limited settings

Despite medical advancements to eradicate human immunodeficiency virus (HIV) around the world, HIV is still among the top leading causes of death in untreated newborn in resourcelimited countries. To address this issue, a confirmatory HIV infection test is required as soon as the baby is born to benefit from available HIV therapies such as antiretroviral therapy1.  However, the presence of maternal HIV antibodies transferred during…


Plasmonic Fluorescence Enhancement in Single Molecule-Based Detection with Nanoantenna

Over the last decade, the field of single-molecule detection has grown rapidly because technical and methodological developments increased sensitivity and gave access to biological processes not observable before. Single molecule detection unveils the short-lived intermediates, the heterogeneous behavior of individual enzymes and stochastic multistep processes like protein folding.1 Currently, single-molecule detection mostly relies on…


Threshold Activation Tandem Mass Spectrometry Coupled to Capillary Electrophoresis for the Structural Analysis of Glycosaminoglycan Mixtures


Explanation Construction and Affective Characteristics in an Active Learning Preparatory Chemistry Course


Probing QOOH-Mediated Reactions in Cyclohexene Oxidation using Photoionization Mass Spectrometry

Combustion research aims to produce fundamental science that enables the development of advanced technologies for cleaner-burning and more-efficient energy conversion, including next-generation internal combustion engines. One primary target is understanding complex chemistry at low-temperature oxidation conditions (< 1200 K) where the formation of NOx is avoided. The chemistry at lower temperature is driven by organic peroxy radicals (ROO),…


Affibodies: Synthetic Protein Binders for Imaging HER2-Positive Breast Cancers

HER2-positive breast cancers affect roughly 25% of patients and are extremely aggressive. While treatments specifically targeting the HER2 receptors are quite successful, they require an accurate diagnosis of HER2 overexpression. Current detection methods of immunohistochemical staining (IHC) and fluorescent in situ hybridization (FISH) do not give a global view of the cancer. They are limited to the metastases biopsied and may lead to…


Nanoparticle-laden Macrophage as a Tumor Tropic Drug Delivery System for Improved Cancer Treatment

Due to poor delivery efficiency, conventional chemotherapy usually requires high injection dose to achieve significant localized control of cancer, which unavoidably leads to systemic toxicity to normal tissues. The emerging nanotechnologies offer new opportunities in optimizing the therapy by altering the post-administration biodistribution of therapeutics and the interaction with the system1. However, the improvement on delivery efficiency…


Development of Molecular Probes for Viral and Bacterial Pathogen Detection Utilizing the Localized Surface Plasmon Resonance (LSPR) of Gold Nanoparticles


Recent developments of 3D printing and applications in microfluidic analysis

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