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Measuring Isotope Incorporation in Proteins Produced Using Uniform or Sparse Labeling with 13C and 15N

Portrait of graduate student Elijah Roberts, speaker
Elijah Roberts
Graduate Student, Department of Chemistry
University of Georgia
iSTEM Building 2, Room 1218
Analytical Seminar

Sparse labeling is a strategy for isotopic enrichment in proteins grown in mammalian systems, such as glycoproteins. We have developed a customized isotope simulator that can simulate enriched peptide and protein isotope patterns. Using this simulator, we have developed a software to experimentally determine the abundance of 13C and 15N in enriched peptides and proteins using isotope patterns and mass information from conventional mass spectrometers. In addition, we have developed software to determine the percent incorporation of isotope enrichment in sparsely-labeled proteins, in conjunction with isotopic fine structure measurements and MS/MS to assess where incorporation is incomplete or metabolic scrambling has occurred.

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