Tags: Analytical Seminar


Validation and Application of a UV-Vis Broadband Cavity-Enhanced Spectrometer for Aerosol Extinction Measurement

Atmospheric aerosols absorb and scatter solar radiation, thereby altering the flux of solar energy reaching Earth. Quantifying the aerosol-radiation interaction is difficult and the effect of aerosols on the propagation of sunlight through the atmosphere is poorly characterized. Accurate, precise observations of aerosol absorption and scattering are necessary to improve radiative transfer models. We have developed a broadband cavity-enhanced…


Electrochemical Sensors for Sensitive Detection of Urea Based on Moleculary Imprinted Polymers

Urea is an essential substance in biological species and the environment. It acts as an direction indicator for abnormal conditions of body and participates in the environmental system as a fertilizer which can penetrate into soil1. A molecularly imprinted polymer (MIP) electrochemical sensor was developed for the detection of urea with a high linear sensitivity, lower detection limit, and good selectivity2. Electrochemical, such as cyclic…


Establish enzyme complex with polymer and DNA scaffolds to improve enzyme catalytic activity and thermal stability

Enzymes are natural catalysts that orchestrate metabolic processes in live cells. Because of their high efficiency, selectivity, and biocompatibility, the applications of these catalysts were extended to many industrial and biomedical technologies for chemical synthesis, biofuel production and food industries. Here I will present my research on modification of lysozyme and cellulases with polymer and DNA scaffolds, where the enzyme complex would…


Fibroblast-Activation-Protein-Targeted Ferritin-Mediated Photodynamic Therapy

Ferritin is an iron storage protein found in numerous species including human beings. Each ferritin consists of 24 subunits which self-assemble into a 12-nm cage, with an 8-nm cavity. The pH-dependent dissociation and re-association of monomers provides a facile approach to encapsulate therapeutics into ferritin cages. Surface modification can also be readily achieved by either chemical conjugation or genetic engineering. These properties, along…


Instrumental Methods for the Field Detection of Fentanyl

Fentanyl is a synthetic opioid prescribed for pain management, but has been increasingly used as an adulterant in illicit substances. Fentanyl is harmful enough that 2 milligrams can cause an overdose, and has been recently implicated in the sharp increase of drug-related deaths in the United States. Current field detection methods utilize color tests. These methods have inherent bias and can result in false positives. They also require on-site…


Development of Electrochemical Atomic Layer Deposition (E-ALD) Methods for IV-VI Semiconductors

Electrochemical atomic layer deposition( E-ALD) is a technique pioneered by the Stickney research group. E-ALD makes use of underpotential deposition to deposit surface limited amounts of material, improving control and quality of thin films.1 In order to create a successful deposition sequence, cyclic voltammetry studies are done to find appropriate deposition parameters. Choice of solution electrolyte and pH have major impact on the deposited…


Combustion aerosols: A complex story of air quality and climate

Vehicles, power plants, agricultural burning, and forest fires are examples of combustion sources whose particulate emissions – known as particulate matter (PM) or aerosols – have profound yet poorly characterized effects on public health and climate. In this seminar, I will present an overview of the state of science of the environmental impacts of combustion aerosols and highlight some of the knowledge gaps. I will then describe the research…


The Structural Role of N-Glycosylation in interactions between Antibodies and Receptors

Immunoglobulin G1 (IgG1) is the most abundant circulating human antibody and also the most common scaffold for therapeutic monoclonal antibodies (mAbs). The destruction of IgG-coated targets by cell-mediated pathways begins with an interaction between the IgG Fc region and multiple varieties of membrane-bound Fc g receptors (FcgRs) on the surface of leucocytes. This interaction requires the presence of an asparagine-linked (N-)glycan…


Leveraging structural biology to dissect calcium modification of ion channel function

In response to changes in calcium concentration, the intracellular calcium sensing protein calmodulin (CaM) alters the function of many proteins throughout the body. Many of these processes are important for normal physiology. Loss of function, miss-function, or untimely CaM function can cause or contribute to disease; some of which can be life threatening. There is a growing appreciation for the wide range of conformations and binding…


Exploration of the Bacterial Lipidome using Hydrophilic Interaction Chromatography and Ion Mobility-Mass Spectrometry

The bacterial lipidome has recently gained appreciation as an important factor in antibiotic resistance and host-pathogen interactions during infection. However, the study of bacterial lipids can be particularly challenging due to the presence of lipid species that are unique to bacteria in general, as well as several lipid classes that are unique to individual species of bacteria. Ion mobility-mass spectrometry (IM-MS) is a rapid gas-phase…
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